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Promega’s MethylEdge™ Bisulfite Conversion System

Promeg'as DNA Methylation Workflow





Promega’s  MethylEdge™ Bisulfite Conversion System is a fast and efficient method for DNA methylation analysis.

The MethylEdge™ System provides an efficient method to rapidly perform bisulfite conversion, giving you more intact, converted DNA for downstream applications. Use the MethylEdge™ System with other DNA Methylation Workflow reagents to perform sensitive DNA methylation analysis on many sample types.

Promega’s MethylEdge™ Bisulfite Conversion System Video


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Shining a Light on Science Misconceptions

The Scientific American Journal News this month talks about seven misused science words that include Hypothesis, Theory, Model, Skeptic, Nature vs. Nurture, Significant, and Natural.
Words like these are frequently misunderstood by students in middle school and high school who are learning them incorrectly. Knowing these words is the difference between knowing what is an absolute truth and what is a lie. According to science, words are an evolutionary continuum and scientists are continually building their understanding with each new invention or experiment.

According to Kat Friedrich of Boston, “Correcting science misconceptions requires a sophisticated understanding of both communication and science. Investing more resources in science education can help improve public science literacy. But scientists may also need to consider using alternate words for concepts that most people misunderstand.”


Scientific American
CITRL Network

Kat Friedrich

When scientists describe how non-specialists misunderstand their language, there’s often a note of sadness in the discussion. If only the United States public was more enlightened than it is today, some bloggers say, then people would understand the language of science. 

A recent Scientific American blog post described how non-scientists in the United States misunderstand the scientific meanings of words like “theory,” “significant,” “hypothesis” and “natural.” A post on the Science 2.0 website provides a longer list of words that are often misunderstood.

What’s wrong with hoping the public will understand scientific language someday? Nothing. But we live in a society where scientists are a specialized group, often socially distant from many of the people who misunderstand them. If scientists want to eradicate misunderstandings and strengthen public awareness of the value of science, better communication and more social interaction is the best solution.

There are tips available online for…

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The immortal HeLa genome

Looking for a good read? Then check out The Immortal Life of Henrietta Lacks! This book is an amazing biography and journey of the HeLa Gemone and a favorite book among many life scientists.

Musings of a mitochondriac

Henrietta Lacks could never have imagined that tiny, invisible parts of her would enthrall, empower, and perplex scientists for decades. In 1951, she developed a particularly aggressive cervical cancer, which unfortunately could not be treated – Henrietta died just a few months later. But her cells lived on, becoming arguably one of biology’s most valuable tools for decades to come.

Yet until now, our knowledge of the genetics of these so-called HeLa cells was rather hazy. I’m happy to be part of the team that has just published the first genome sequence of a HeLa cell line. (We were a little shocked by the amount of coverage our press release generated, even including a NatureNews feature!) Here I’d like to explain why we did this and what we learned – and perhaps also importantly, what we did not learn.

(Part 2 of this post will be an interview…

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CDC Warns Medical Professionals Must Prepare To Combat Rare, Potentially Deadly Superbug

super bug







Health care settings like hospitals and nursing homes need to make preparations to prevent the spread of a rare “superbug,” the CDC is warning. There’s recently been a sharp jump in the number of reports of the potentially deadly bacteria, which is resistant to all last-resort antibiotic treatments — and the CDC is prompting health […]/p

via CDC Warns Medical Professionals Must Prepare To Combat Rare, Potentially Deadly Superbug.

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Repairing Protein Might Prevent Cancer Recurrence

We have all had a friend or family member who has died of cancer. If you haven’t, then consider yourself one of the lucky ones. Cancer affects almost everyone in one way or another whether you realize it or not. Now, there may be a repairing protien that can prefent the recurrence of some cancers!

ScienceNews in quotes

Krüppel-like factor 8 (KLF8) regulates critical gene transcription and cellular events associated with cancer. However, the role of KLF8 in cancer remains largely unknown. Here, we report a surprisingly novel role for KLF8 in DNA repair in breast cancer cells. Comet, clonogenic, and WST-1 assays showed that KLF8 expression is required for protecting human breast cancer cells from doxorubicin-induced DNA damage and cell death. Western blotting indicated that overexpression of ectopic KLF8 attenuated the levels of the DNA damage marker γH2A.X in doxorubicin-treated PARP-1(+/+) but not PARP-1(-/-) mouse embryonic fibroblasts, whereas the PARP-1-binding-defective KLF8 mutant failed to do so. Interestingly, in response to the DNA damage, KLF8 was phosphorylated by the DNA-dependent protein kinase catalytic subunit and, subsequently, SUMOylated by SUMO E3 ligases protein inhibitors of activated STAT (PIASs), which depends upon the interaction of KLF8 with DNA-dependent protein kinase catalytic subunit, PIASs, and PARP-1 as well as their enzymatic…

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Leukemia Cure for Emma


NY Times Article “Breakthrough Experimental Treatment for Advanced Leukemia”
“Emma was near death from acute lymphoblastic leukemia but is now in remission after an experimental treatment at the Children’s Hospital of Philadelphia. The experiment, in April, used a disabled form of the virus that causes AIDS to reprogram Emma’s immune system genetically to kill cancer cells.”

A disabled form of the virus that causes AIDS was used to reprogram Emma’s immune system in order to kill her cancer cells. Three adults with Leukemia, who have also had the experimental treatment are now cancer free.  However, the altered T cells do have a drawback: they destroy healthy B-cells as well as cancerous ones, leaving patients vulnerable to certain types of infections, so Emma and the other patients need regular treatments with immune globulins to prevent illnesses. 
Click on the link above to read more about this amazing breakthrough in Science!


NY Times – An immune system trained to kill cancer”

New England Journal of Medicine – Chimeric Antigen Receptor–Modified T Cells in Chronic Lymphoid Leukemia

Science Magazine – Tumor immunotherapy with T lymphocytes